Phytochemical analysis of Nigella sativa and its antibacterial activity against clinical isolates identified by ribotyping
2013
Ishtiaq, S. (National University of Sciences and Technology, Islamabad (Pakistan). Dept. of Plant Biotechnology) | Ashraf, M. (National University of Sciences and Technology, Islamabad (Pakistan). Dept. of Plant Biotechnology) | Hayat, M.Q. (National University of Sciences and Technology, Islamabad (Pakistan). Dept. of Plant Biotechnology) | Asrar, M. (Ministry of Science and Technology, Islamabad (Pakistan). Pakistan Council for Science and Technology)
The study was carried out to find in vitro anti-bacterial activity of Nigella sativa extracts against seven clinical isolates identified by ribotyping. Crude Extracts of N. sativa in eight organic solvents (Aqueous, Methanol, Ethanol, Cloroform, Butanol, Diethyl ether, n-Hexane and Acetone) were evaluated at 5 different concentrations 100 mg/mL, 50 mg/mL, 25 mg/mL, 10 mg/mL and 5 mg/mL by using disk diffusion method against human pathogenic bacterial strains including Gram-positive bacteria (Enterococcus faecalis IARS1, Staphylococuss aureus IARS4) and Gram-Negative bacteria (Acinetobacter junii IARS2, Escherichia coli IARS3, Proteus mirabilis IARS5, Serratia marcescens IARS6, Enterobacter cloacae IARS7). All the organic N. sativa extracts with minimum concentration of 5 mg/mL showed effective growth inhibition against the tested pathogenic bacterial strains. Standard antibiotics ceftriaxone 100 mug/mL, Amoxycillin 100 mug/mL, Gentamycin 100 mug/mL were used as a positive control. Out of eight extracts Methanolic, Ethanolic, Chloroform, n-Hexane and Acetonic extracts showed maximum antibacterial activity. N. sativa extracts were screened for qualitative detection of secondary metabolites and it showed that steroids, tannins, flavanoids, coumarins, cardiac glycosides, saponins and diterpenes were present in methanolic and ethanolic extract of N. sativa. The results of this study will provide an insight to characterize bioactive compounds from these extracts, which can act as strong bacterial growth inhibitor against wide range of infectious disease caused by pathogenic bacteria.
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