Heavy metal and antimicrobial resistant bacteria isolated from Karachi coastal area as an indicator of pollution
Akhter, J. (Karachi Univ. (Pakistan). Centre for Molecular Genetics) | Ahmed, N. (Karachi Univ. (Pakistan). Centre for Molecular Genetics) | Badar, U. (Karachi Univ. (Pakistan). Dept. of Genetics) | Waqar, M. | Shoeb, E.
In the present study 70 marine bacterial isolates were studied in order to speculate about pollution along the Karachi coastal area, through the isolation of heavy metal and antimicrobial resistant bacteria. The study revealed resistance characters in 62% gram positive and 38.3% gram negative bacterial strains. Heavy metal resistance was found in 30% of the total isolates which was mostly against zinc, nickel and chromium. Most of the marine bacterial isolates were found resistant to (beta-lactam (ampicillin) and aminoglycoside (kanamycin and streptomycin). Isolation and identification of these isolates has indicated occurrence of high level of pollution on the Karachi coast. Besides initial characterization through microbiological and molecular techniques, some of the selected strains were also identified through 16S rRNA gene sequencing. The nutshell of the study, analyzing the microbial flora verifies the hypothesis that states high rank pollution along the Karachi Coastline.
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