Chemical and biological evaluation of Cretan medicinal plant infusions: Salvia pomifera, Origanum dictamnus, Laurus nobilis and Pelargonium odoratissimum
2010
Tawfik, A. F.
The aim of this study was to investigate and evaluate the presence of antioxidant activity in the aerial parts of Salvia pomifera, Origanum dictamnus, Laurus nobilis and Pelargonium odoratissimum (origin Crete, Greece). The antioxidant activity, evaluated by the chemiluminescence method and the IC50 for each compound were measured and calculated. The following results were obtained: Geranium odoratissimum exhibited the highest antioxidant activity (IC50= 2.41 plus-minus 0.038 micrograms/ ml), higher than Salvia pomifera (IC50= 2.89 plus-minus 0.121 micrograms/ ml), and Laurus nobilis (IC50=3.82 plus-minus 0.112 micrograms/ ml), while the lowest activity plant was for Origanum dictamnus (IC50=3.90 plus-minus 0.051 micrograms/ ml). The Rancimat method was also employed to evaluate the antioxidant activity of the infusions produced from the above mentioned plant species. In order to obtain the fraction responsible for the antioxidant activity further chromatographic steps were performed: Reversed phase column chromatography and the TLC paper test. Reversed phase column chromatography was undertaken only on the two plants that showed the highest and the lowest antioxidant activity (Geranium odoratissimum and Origanum dictamnus). The obtained fractions selected according to their profile on TLC paper were further tested using the chemiluminescence method.
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