Effect of culture medium on bacteriocin production by Lactobacillus rhamnosus hn001 and Lactobacillus reuteri atcc 53608
2012
Aguilar-Uscanga B. R.,, Universidad de Guadalajara, Jalisco, (México.) | Solís-Pacheco J. R. | Plascencia L. , | Aguilar-Uscanga M. G. | García H. S., Lacroix M.
The aim of this study was to evaluate the effect of media on bacteriocin production by Lactobacillus rhamnosus HN001 and Lactobacillus reuteri ATCC 53608 using three different media: YPM, YPF and MRS supplemented with glucose and K2HPO4. The optimum temperature was 37 deg C and initial pH 6.5. Bacteriocin-like substances produced by tested bacteria in MRS medium supplemented with glucose and K2HPO4 exhibited a broad antimicrobial spectrum determined by well diffusion assay against indicator bacteria Listeria monocytogenes, Lactobacillus sakei, Enterococcus faecium, Lactobacillus delbrueckii, Lactobacillus acidophilus, but no antimicrobial spectrum against E. coli O157:H7, Salmonella typhimurium, Staphylococcus aureus, Bacillus cereus was detected. Bacteriocin was sensitive to protease IV, trypsin, pepsin and alfa-amylases, but resistant to lipase. It was also resistant to detergents such as Tween 80, Triton-X and SDS. This bacteriocin was thermo-stable (resistant at 60 deg C, 90 deg C and 100 degC for 30 min). Tested bacteria showed the best antimicrobial (bacteriocin-like) activity after growth in MRS medium. Bacteriocin substances produced by tested bacteria showed promising thermo-stable technological properties.
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