THE EFFECT OF LIGHT QUALITY ON SOME PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS OF IN VITRO PROPAGATED PETUNIA CV. BLUE STAR PLANTS
2014
Petrova, N., Sofia University St. Kliment Ohridski, Sofia (Bulgaria) | Dimitrova, M., Sofia University St. Kliment Ohridski, Sofia (Bulgaria) | Dragolova, D., Sofia University St. Kliment Ohridski, Sofia (Bulgaria) | Rogova, M., Sofia University St. Kliment Ohridski, Sofia (Bulgaria) | Kapchina- Toteva, V., Sofia University St. Kliment Ohridski, Sofia (Bulgaria)
Petunia hybrida plants were propagated in vitro by single-node explants on basic MS medium 3.0% sucrose, 0.7% agar, pH adjusted to 5.7 – 5.8. The explants were cultivated in a growth chamber at different combinations of monochromic LED lighting at equal irradiance of 70 мmol.m-2.s-1 at the tops of the plants, 12-h photoperiod, 25/25 °C day/night temperatures and 60 – 70% relative humidity. Three different types of LEDs, emitting in the red (635 nm, R), green 531 nm, G, and blue 463 nm, B region of the spectrum are used to compose 66: 33% RB and 33: 33: 33% RGB light. After four weeks of cultivation the following characteristics were observed: stem number and length, rooting, callus formation, fresh/dry weight index and malondialdehyde MDA levels. P. hybrida cultivated on red/blue LED form higher number and longer shoots and higher fresh/dry weight index they also have slightly larger content of MDA than plants exposed to cool white light.
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