Root-knot nematode Cg-1 and its homologous genes cloning and sequences analysis | 2种根结线虫Cg-1及其同源基因的克隆和序列分析
2013
Wu Ping, South China Agricultural University, Guangzhou (China), Laboratory of Plant Nematology | Zhuo Kan, South China Agricultural University, Guangzhou (China), Laboratory of Plant Nematology | Luo Mei, South China Agricultural University, Guangzhou (China), Laboratory of Plant Nematology
Chinese. 采用同源克隆的方法，首次从能克服Mi-1抗性的象耳豆根结线虫（Meloidogyne enterolobii）中克隆了Cg-1同源基因，同时克隆了一个爪哇根结线虫无毒种群的Cg-1及其同源基因。基因序列比较结果显示，象耳豆根结线虫中不存在Cg-1基因，即象耳豆根结线虫中只具有不包含CAATGA片段的Cg-1同源序列，进一步表明Cg-1可能是一个无毒基因。对Cg-1基因序列分析的结果表明，Cg-1基因可能是通过其第三个开放阅读框（ORF）编码的多肽或结构性mRNA或两者共同行使生物学功能。
Show more [+] Less [-]English. Cg-1, with 8 homologous genes, from Meloidogyne javanica is a candidate avirulence gene corresponding to tomato resistance gene Mi-1. Comparing with its homologs, Cg-1 contains a CAATGA fragment insertion. In this study, Cg-1 homologous clones from Meloidogyne enterolobii, a root-knot nematode that is able to overcome tomato Mi-1 resistance, were firstly cloned. Meanwhile, Cg-1 and its homologous clones of an M. javanica avirulent strain in our laboratory were cloned. Comparison of Cg-1 and these homologous clones from M. enterolobii and M. javanica strains showed that Cg-1 gene was inexistent in M. enterolobii that is there were only Cg-1 homologous genes which did not contain CAATGA existing in M. enterolobii. This result further indicated that Cg-1 may be an avirulence gene. Sequence analysis of Cg-1 gene predicts that the third open reading frame (ORF) encoding peptide and its structural mRNA, or both exercise biological function of Cg-1.
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