Colchicine-induced autotetraploids of five cassava cultivars | 秋水仙素诱导获得5个木薯品种的同源四倍体植株
2013
Nie Yangmei, Huazhong Agricultural University, Wuhan (China), College of Horticulture and Forestry Sciences | Wen Feng, Huazhong Agricultural University, Wuhan (China), College of Horticulture and Forestry Sciences | Guo Wenwu, Huazhong Agricultural University, Wuhan (China), College of Horticulture and Forestry Sciences
Chinese. 为获得纯合稳定的木薯（Manihot esculenta Crantz）同源四倍体，以不同质量浓度（0.1、0.3、0.5g/L）的秋水仙素和时间（3、6、9h）组合对5个木薯品种的腋芽进行诱导处理，然后转入MS培养基培养再生试管苗，采用流式细胞仪和根尖压片技术鉴定其倍性，筛选出四倍体。经多代无性繁殖，结果显示5个品种均获得稳定的四倍体植株。将木薯Col22和华南8号的四倍体移入温室，5个月后与对照二倍体相比，其叶片变宽、变厚，气孔的长度、宽度、密度等方面均有显著差异。同源四倍体新种质的创制为进一步用于木薯品种改良提供了珍贵的试材。
Show more [+] Less [-]English. Using five cassava cultivars as explants, different colchicine concentrations (0.1, 0.3, 0.5 g/L) and time duration of treatment (3, 6, 9 h) were combined to induce autotetraploids of cassava in vitro using axillary buds. Generated shoots were transferred onto the basic MS medium for further development. Ploidy analysis via both flow cytometry and root tip squash technique verified that recovered plantlets from all five cultivars were stable autotetraploids after several subcultures. Autotetraploid plantlets originated from Co122 and SC8 and its corresponding parental plants were transferred into green-house. Phenotypes of autotetraploids were comparatively analyzed after five-month growth. Results showed that leaves of autotetraploids became significantly wider and thicker than those of parental plants. The length and width and density of stomas had significant difference between the control and autotetraploids. These novel autotetraploids have great potential for the further genetic improvement of cassava.
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