Molecular and phenotypic analysis on effect of over-expression and RNA interference of a transcriptional factor gene SUSIRI on transgenic rice | 水稻转录因子基因SUSIRI的过表达及RNA干扰对水稻分子及表型效应的分析

Chinese. 在克隆到水稻转录因子基因SUSIRI的基础上，构建其过表达及RNA干扰载体进行水稻的遗传转化。由潮霉素筛选及分子检测得到的T0代植株经大田栽植，共有34株过表达及46株RNA干扰植株获得T1代转基因种子。继续种植T1植株收获T2代种子，同时用半定量RT-PCR分析苗期及穗期野生型水稻中SUSIRI基因的时空表达谱并比较过表达及RNA干扰植株的SUSIRI基因在叶片及幼穗中的表达状况，发现过表达植株与野生型对照的基因表达基本相同，但RNA干扰植株的表达受到明显抑制。对过表达及RNA干扰植株稻穗性状考察表明：与野生型对照相比，无论是过表达还是RNA干扰，转基因植株的穗粒数、结实率降低，RNA干扰植株表现得尤为严重（P0.05），但对水稻谷粒粒重的影响并不显著（P0.05）。SUSIRI基因的过表达未出现使稻穗性状明显改良的株系，而RNA干扰会导致水稻的结实能力严重降低。

English. Based on cloning a transcriptional factor gene SUSIRI from rice, its transgenic vectors of both over-expression and RNA interference were constructed for rice transformation. Through hygromycin selection and molecular detection, T0 transgenic plants were grown to obtain the T1 seeds from 34 over-expressing plants and 46 RNA plants. When T1 was planted for T2 harvest, the spatial and temporal expression of SUSIRI was analyzed with semi-quantitative RT-PCR in wild type rice and the expressing profile in leaf and panicle was compared with that of over-expressing and RNA interfering transgenic rice. It was found no difference in over-expressing plant, but the severe inhibition of SUSIRI expression occurred in RNA interfering transgenic rice. Investigation of panicle traits indicated that the number of grains per panicle and seed setting rate in all transgenic rice plant decreased as compared to wild type, and the difference at significant level (P0.05) was frequent in RNA interfering transgenic rice. However, 1 000-grain weight among three varieties of rice had no significant difference (P0.05). The over expression of SUSIRI gene had no obvious improvement of panicle traits in rice, but the RNA interference could result in the serious damage of seed-setting ability of rice.