Screening similar varieties of rapeseed (Brassica napus L.) in DUS testing using SSR markers | 利用SSR标记筛选DUS测试中甘蓝型油菜近似品种

Chinese. 随着分子标记技术的迅速发展，将分子标记运用于辅助植物新品种保护中的特异性、一致性和稳定性（distinctness，uniformity，stability，DUS）测试成为热门话题。为了探讨SSR标记用于甘蓝型油菜近似品种筛选的可行性，本研究以26份自交系和54份2008-2009、2009—2010和2010—2011年DUS测试品种为研究材料，从952对SSR引物中筛选出49对多态性丰富、带型清晰、易以统计的核心引物。49对引物共检测到57个位点311个等位变异，每个位点包含等位变异数变幅为2～13个，平均5.5个，平均多态性信息含量（polymorphism information content，PIC）为0.53。聚类结果表明，在遗传相似系数为0.93时，该套核心引物可将所有参试品种区分开，同一育种单位或地区的品种优先聚为一起，聚类结果与品种系谱基本吻合。说明SSR标记可用于DuS测试中甘蓝型油菜近似品种筛选。

English. With the rapid development of molecular techniques, molecular markers used in assisting DUS testing have become a hot topic. In order to exploring feasibility of screening similar varieties of rapeseed (Brassica napus L.) using SSR markers, 80 accessions including 26 inbred lines and 54 testing varieties identified from the year of 2008 to 2011 were employed in this study. According to the richness of polymorphism information content (PIC), the clarity of PCR bands as well as the easy discrimination of different alleles, 49 pairs of SSR primers was screened from 952 pairs of primers of SSR. Total 311 alleles at 57 loci could be identified via 49 pairs of primers. The number of alleles per locus ranged from 2 to 13 with an average of 5.5, and the mean value of polymorphism information content was 0.53. Clustering analysis showed that all tested accessions were completely differed from each other by using 49 markers based on the threshold of genetic similarity coefficient at 0.93. The varieties coming from the same breeders or regions were clustered together at first. In addition, clustering results was basically consistent with the pedigree of variety. In this research we would have conclusion that using SSR markers as an assisting selection marker to screen similar varieties is feasible in DUS testing.