Development of microsatellite markers for identifying Misgurnus anguillicaudatus and Paramisgurnus dabryanus in juvenile stage | 微卫星标记快速鉴别幼鱼阶段的泥鳅与大鳞副泥鳅
2013
Chen Gangming, Huazhong Agricultural University, Wuhan(China), College of Fisheries/Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Laboratory of Freshwater Animal Breeding ,Ministry of Agriculture | Wang Weimin, Huazhong Agricultural University, Wuhan(China), College of Fisheries/Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Laboratory of Freshwater Animal Breeding ,Ministry of Agriculture | Cao Xiaojuan, Huazhong Agricultural University, Wuhan(China), College of Fisheries/Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Laboratory of Freshwater Animal Breeding ,Ministry of Agriculture
Chinese. 从DDBJ数据库中选取已发表的47个泥鳅微卫星标记,在采自湖北省的大鳞副泥鳅群体中进行跨种扩增,发现有44个微卫星标记能在该群体获得扩增产物,另外有3个微卫星标记（即Mac31、Mac63和Mac541）完全不能获得扩增产物。以湖北省泥鳅和大鳞副泥鳅自交子代幼鱼DNA为模板,采用Mac31、Mac63和Mac541这3个微卫星标记的引物对进行PCR扩增,结果显示在以泥鳅自交子代幼鱼DNA为模板的体系中能扩增出目的条带,在以大鳞副泥鳅自交子代幼鱼DNA为模板的体系中完全不能扩增出目的条带。因此,Mac31、Mac63和Mac541这3个微卫星标记可用来鉴别幼鱼阶段的泥鳅与大鳞副泥鳅。
Show more [+] Less [-]English. Forty-seven Misgurnus anguillicaudatus microsatellite markers were randomly selected from DDBJ database and used for cross-species amplification in a population of Paramisgurnus dabrya- nus from Hubei Province. Forty-four pairs of primers amplified P. dabryanus DNA, while 3 primers in- cluding Mac31, Mac63 and Mac541 failed. Using genomic DNA of selfing offsprings juveniles of M. an- guillicaudatus and P. dabryanus as templates, PCR amplifications were performed using three primers of Mac31 ,Mac63 and Mac541. The results showed that target bands were detected only in selling off- springs of M. anguillicaudatus,suggesting that markers of Mac31, Mac63 and Mac541 are useful for i- dentifying M. anguillicaudatus and P. dabryanus at the juvenile stage.
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