Requirements for essential amino acids of Barbus capito | 大鳞鲃幼鱼氨基酸需要量

Chinese. 选择体质量为（11.29±0.07）g的健康大鳞鲃,共分2个处理组（蛋白饲料组和无蛋白饲料组）,每个处理3个重复,每重复20尾鱼。根据试验前后鱼体氨基酸变化分别得到大鳞鲃日增加氨基酸和日维持氨基酸需要量,并根据两者之和得到氨基酸日需要量。结果表明：1）从大鳞鲃生长状况看,与蛋白饲料组相比,无蛋白饲料组鱼体末质量及特定生长率均显著降低（P〈0.05）。2）从体成分变化情况看,与蛋白饲料组相比,无蛋白饲料组鱼体水分含量显著升高（P〈0.05）。3）如大鳞鲃饲料蛋白水平为43%,饲料利用率为90%,日投饵率为3%,大鳞鲃氨基酸日需要量为（mg/100g）：苯丙氨酸25.54,蛋氨酸15.80,精氨酸36.08,赖氨酸53.76,亮氨酸49.95,苏氨酸25.05,缬氨酸25.99,异亮氨酸25.33,组氨酸19.94;大鳞鲃饲料中氨基酸含量为（g/100g）：苯丙氨酸0.85,蛋氨酸0.53,精氨酸1.20,赖氨酸1.79,亮氨酸1.67,苏氨酸0.84,缬氨酸0.87,异亮氨酸0.84,组氨酸0.66。

English. This study was conducted to estimate essential amino acids （EAA） requirements of ju- venile Barbus capito. Feeding experiments of B. capito （（11.29±0.07）g） were conducted with the pro- tein diets （PD） and protein-free diets （FPD） and each diet was randomly assigned in triplicate. The EAA requirements of B. capito were determined based on the daily increment and daily maintenance require- ment of each EAA in fish body. Compared with the PD group, the final weight and specific growth rate decreased significantly （P 〈 0. 05） and the whole body moisture content increased significantly （P〈0. 05） in the FPD group. When dietary protein level was 43%, feed utilization rate was 90% and feeding rate was 3%,the daily EAA requirements of juvenile B. capito （mg/100 g body weight） were as follows.phenylalanine 25. 54, methionine 15. 80, arginine 36.08, lysine 53. 76, leucine 49. 95, threonine 25.05,valine 25.99,isoleuline 25.33 and histidine 19.94. The requirements of EAA for B. capito feed （g/100 g） were as follows., phenylalanine 0. 85, methionine 0. 53, arginine 1. 20, lysine 1. 79, leucine 1.67,threonine 0.84,valine 0. 87,isoleuline 0. 84 and histidine 0. 66.