Study of different concentration of growth regulators on micro propagation of two commercial and one wild Rose.
2013
Beyramizadeh, Ebrahim | Safari, Akram | Edricy, Behzad | Banijamali, Sayd Mohamad
Rose is one of important cut flower in the world and one of propagation methods of this flower is tissue culture and using of auxiliary bud as explants. At this research were studied micro propagation of two commercial cultivars and one wild rose. In first experiment were studied micro propagation of two commercial rose cultivar Vandentta (red) & Leandera (cream-yellow) and in second experimental one wild rose. After growth of explants in initiation media shoots were cultured in proliferation media including MS medium with BA & NAA. All experiments were done by factorial experiment based on Complete Randomized Design (CRD). The result of analysis variance showed that effect of BA was significant at 1% level for number of shoot and height of shoot. The best proliferation was done for Vandetta at 2 mg/l BA with 0.1 mg/l NAA, for Leandera 2 mg/l BA with 0.01 mg/l NAA, in Wild Rose 2 mg/l BA with 0.5 mg/l Kin. Different level of IBA NAA and IAA was used for rooting. The best rooting was showed 1.22 mg/l IBA in Wild Rose and 0.2 mg/l NAA with 0.25 mg/l IBA in Vandenta. All of plantlet were hardening and were transferred in commercial rose greenhouse. Key word: Rose ,Micro propagation ,Auxiliary bud ,Proliferation ,Rooting
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