Development of an ELISA for determination of potency in poly valent snake anti venoms
2012
Rasool Madani
The venom of Naja Naja Oxiana, Vipera Lebetina, Vipera Albicornouta, Echis Carinatus, Pseadocerastus Persicus and Agkistrodon Halys snakes are used to determine the potency of the polyvalent sera in neutralizing the venom. LD50 value per mouse was determined 6.5 ¦Μg. Netralization methods for obtaining potency of anti venom are competitive ELISA (Enzyme linked immunosorbent assay) and in vivo assay (ED50). Both of these tests were performed to estimate the serum potency, in 15 samples of hyper-immune equines. Results of competitive ELISA showed that at 1/١٢000 dilution of serum can inhibits 50 percent of antigens. Five fold of LD50 as challenge dose of venom for lethality neutralization test was applied for all serums. Competitive ELISA was compared with current biological assay ED50. Significant correlations between ELISA titers and values of ED50 at level of P΅ά 0.01 and r=0.9% was observed, that indicates competitive ELISA can estimate antibody neutralizing capacity of the serum as well as the in vivo assay. The results of present study shows C-ELISA that measures Ag-Ab complex can used as a suitable replacement method for lethal neutralizing in vivo method. MINISTRY
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