Comparison of the pre-inflammatory cytokin changes and their relationships with hematological parameters in healthy and infected cows with Theileria annulata in Urmia

Theileriosis is one of the erythrocyte and lymphocyte parasitic disease in cows which characterized by fever, jaundice, anemia and death. Theileriosis due to Theilaeria annulata is the results of an inflammatory response in animals body that directly related to pre-inflammatory parameters. The amounts of these parameters were investigated in 26 and 68 healthy and infected cows to Theilaeria annulata, respectively, in Urmia 2012. Theileria infection was confirmed in the clinical and parasitological examinations and parasitemia were determined in peripheral blood smear. Jugular whole blood were prepared to evaluate hematocrit, hemoglobin, white cell count (WCC), differencial count, and serum for TNF΅ IL1B and IL3. The results were compared with healthy and non- parasitemic cows. The number of 1-5, 6-10 and 11-20 infected erythrocytes was classified as benign, moderate and severe parasitemia, respectively. The number of benign, moderate and severe parasitemin were 53 (77.95%), 9 (13.3%) and 6 (8.8%), respectively. Chi-Square test showed significant differences (P0.05) among 3 groups with the highest and lowest infection were found in benign and severe parasitemia. The lowest percentage of PCV and Hb concentration and highest concentrations of TNF΅ IL1B, IL3 and leucocytes were found in severe infection to theileria, but monocytes were high in moderate group. The lowest leucocytes monocytes and low concentration of TNF΅ IL1B and IL3 were observed in benign infection. Mean PCV and Hb decreased, while WCC, TNF, IL1B and IL3 increased from benign to severe infection. ANOVA showed significant differences in lymphocytes, monocytes (P0.05), TNF, IL1B and IL3 (P0.01) from benign to severe infection. The minimum concentrations of TNF, IL1B and IL3 were in control animals and the maximum in severe infected cows. There were significant negative correlations between PCV, Hb and pre-inflammatory parameters and significant positive correlations among TNF, IL1B and IL3 (P0.01). With the exception of PCV and Hb pre-inflammatory parameters were correlated with blood parameters. The highest correlations were observed among pre-inflammatory parameters (r