Diagnosis of canine brucellosis using recombinant ribosomal protein L7/L12
2012
Lee, H.K., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Kim, J.W., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Ha, Y.M., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Her, M., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Kim, J.Y., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Lee, K.C., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Kang, S.I., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Jung, S.C., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea
Brucella (B.) canis is mainly transmitted by direct or indirect contact with aborted fetuses and placenta. It's also known to be able to infect human, which likely results in providing veterinarians and companion animal owners for infectious risk. To develop diagnostic ELISA, we cloned and expressed rp1L gene of B. canis, which encodes the ribosomal protein L7/L12. Using this purified recombinant protein, indirect-ELISA (iELISA) was evaluated using 78 positive and 44 negative sera. The sensitivity and the specificity of iELISA were 94% and 89%, respectively. The results indicated that indirect-ELISA using recombinant ribosomal protein L7/L12 may be useful for diagnosis of canine brucellosis.
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