Specific Monoclonal Antibodies against Paenibacillus larvae Flagella and Spores
2013
Kim, S.Y., Jeju National University, Jeju, Republic of Korea | Kim, C.H., Jeju National University, Jeju, Republic of Korea | Kim, J.H., Jeju National University, Jeju, Republic of Korea | Son, W.G., Jeju National University, Jeju, Republic of Korea | Hwang, K.K., Jeju National University, Jeju, Republic of Korea | Lim, Y.K., Jeju National University, Jeju, Republic of Korea
For the control of the American foulbrood disease (AFB), a rapid and accurate diagnosis must be preceded. The immunological diagnostic methods could be applied to the inspection of bulk samples in laboratories, as well as rapid test in the field. Specific antibodies is the key of immunological diagnosis. We prepared flagella and spores antigens of Paenibacillus larvae and immunized into mouse to obtain monoclonal antibodies against each different antigens. Four of monoclonal antibodies against flagella and 4 of them against spore showed no cross reactivity not only to each other antigens but also with Mellisococcus plutonius, the causative agent of Europian foulbrood disease. Providing suitable combination of antibodies pair, the monoclonal antibodies developed in this study could be applied in development of ELISA and Immunochromatographic assay for the diagnosis of AFB in honey bee.
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