Analysis of Genetic Polymorphism of Korean Ginseng Cultivars and Breeding Lines using RAPD Markers
2013
Bang, K.H., RDA, Eumseong, Republic of Korea | Chung, J.W., RDA, Suwon, Republic of Korea | Kim, Y.C., RDA, Eumseong, Republic of Korea | Cho, I.H., RDA, Eumseong, Republic of Korea | Kim, J.U., RDA, Eumseong, Republic of Korea | Shin, M.R., RDA, Eumseong, Republic of Korea | Hyun, D.Y., RDA, Eumseong, Republic of Korea | Kim, D.H., RDA, Eumseong, Republic of Korea | Cha, S.W., RDA, Eumseong, Republic of Korea | Kim, K.H., RDA, Eumseong, Republic of Korea | Moon, J.Y., MIFAFF, Seoul, Republic of korea | Noh, B.S., Seoul Women University, Seoul, Republic of Korea | Kim, H.S., Chungbuk National University, Cheongju, Republic of Korea
In this study, Random Amplified Polymorphic DNA (RAPD) analyses were used to clarify the genetic polymorphisms among Korean ginseng cultivars and breeding lines and to classify them into distinct genetic groups. Among 130 primers in RAPD analysis, 25 primers were selected as the appropriate identification of genetic characteristics in Korean ginseng cultivars and breeding lines. Twenty-five RAPD loci generated a total of 189 DNA bands. Amplified PCR products were showed the highly reproducible banding patterns at 100 ~ 2,800 bp. The number of amplified bands for each RAPD primers ranged from 3 to 17 with a mean of 7.6 bands. In the case of OPD19 primer, 5 banding patterns were displayed in Korean cultivars and breeding lines. Banding patterns were approximately 500 to 1,300 bp amplicons. The number of alleles for each RAPD locus ranged from 1.33 to 2.00 with a mean of 1.71 alleles. OPD15 and OPF02 primers demonstrated the highest and the lowest genetic polymorphisms, respectively. Cluster analysis based on genetic similarity estimated by RAPD markers classified Korean cultivars and breeding lines into 2 groups. Group I included Gopoong and 11 breeding lines (50%); group II included 3 breeding lines (1.3%). Consequently, the results of ginseng-specific RAPD markers may prove useful for the evaluation of genetic diversity and differentiation of Korean ginseng cultivars and breeding lines.
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