Phylogenetic Analysis of Korean Native Aster Plants Based on Internal Transcribed Spacer (ITS) Sequences
2012
Hong, S.Y., Highland Agriculture Research Center, NICS, RDA, Pyeongchang, Republic of Korea | Cho, K.S., Highland Agriculture Research Center, NICS, RDA, Pyeongchang, Republic of Korea | Yoo, K.O., Kangwon National University, Chuncheon, Republic of Korea
This study was carried out to decide ITS (internal transcribed spacer) sequence of some Korean native Aster species and to resolve their relationship among Korean native Aster, including Kalimeris, Gymnaster, Heteropappus genus separated from Aster in a previously study based on the pappus length. We registered 11 ITS sequences of Aster species including A. glehni to GenBank and those sequences were used for the cluster analysis with Kalimeris species. The size of ITS1 was varied from 248 to 256 bp, while ITS2 was varied from 220 to 222 bp. The G + C content of the ITS region ranged from 49.4 to 53.5%. Pairwise comparison results showed that the substitution rate of ITS1 and ITS2 region was 9% and 10%, respectively. Kalimeris sensu strict substitution rate was lower than that of Aster sensu strict species. The strict consensus parsimonious cluster analysis showed A. tripolium is the first branching from the clade and the next is A. scaber. The Kalimeris species except for the A. hispidus were grouped into the same clade with high bootstrap value (91%) within Aster. Gymnaster and Heteropappus that has been classified by morphological characters were also grouped into broad sense Aster clade. These results implied these three genera could be merged together into Aster based on the ITS sequences.
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