A Set of Allele-specific Markers Linked to L Locus Resistant to Tobamovirus in Capsicum spp.
2012
Lee, J.D., Pepper and Breeding Institute, Business Incubator, Seoul National University, Suwon, Republic of Korea | Han, J.H., Pepper and Breeding Institute, Business Incubator, Seoul National University, Suwon, Republic of Korea | Yoon, J.B., Pepper and Breeding Institute, Business Incubator, Seoul National University, Suwon, Republic of Korea
The resistance to Tobamovirus in Capsicum spp. has been known to be controlled by five different alleles (L∨0, L¹, L², L³, and L⁴) of L locus on the telomere of long arm of pepper chromosome 11. To develop a set of molecular markers differentiating all the alleles of L locus, we used five pepper differential hosts including Capsicum annuum Early California Wonder (ECW, L∨0L∨0), C. annuum Tisana (L¹L¹), C. annuum Criollo de Morelos 334 (CM334, L²L²), Capsicum chinense PI 159236 (L³L³), and Capsicum chacoense PI 260429 (L⁴L⁴). Developing a series of CAPS or SCAR markers specifically linked to the alleles was allowed by the sequence comparison of PCR amplicons of the L³-linked markers (189D23M, A339, and 253A1R) and BAC sequences (FJ597539 and FJ597541) in the pepper differentials. Genotypes deduced by these markers in 48 out of 53 F₁ hybrids of commercial pepper varieties were consistent with their phenotypes by bioassay using Tobamovirus pathotypes (P∧0, P₁, and P∧1,2). Consequently, these markers can be useful to differentiate L alleles and for breeding Tobamovirus resistance in pepper with marker-assisted selection.
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