Growth Characteristics of in Vitro Mass Propagated Plantlets of Ever-bearing Strawberry 'Goha' according to Aeration Rate in Bioreactor
2012
Kim, H.J., Highland Agriculture Research Center, NICS, RDA, Pyeongchang, Republic of Korea | Lee, J.N., Highland Agriculture Research Center, NICS, RDA, Pyeongchang, Republic of Korea | Kim, K.D., Highland Agriculture Research Center, NICS, RDA, Pyeongchang, Republic of Korea | Im, J.S., Highland Agriculture Research Center, NICS, RDA, Pyeongchang, Republic of Korea | Lim, H.T., Kangwon National University, Chuncheon, Republic of Korea | Yeoung, Y.R., Gangneung-Wonju National University, Gangneung, Republic of Korea
This study was conducted to determine the optimal aeration rate for mass propagation of ever-bearing strawberry by bioreactor culture. The aeration rate was treated in four levels: 0.1 vvm (air volume/medium volume/min), 0.2 vvm, 0.3 vvm, and 0.4 vvm. In 0.2 vvm conditions, shoot length was the longest at 9.03 cm in bioreactor culture, leave numbers were 40.4 ea and fresh weight was 6,106 mg. Plant growth rate at 0.2 vvm condition was faster than other treatments. In the aeration condition, 0.2 vvm was most effective to increase aerial part growth and to decrease medium consumption. As the culture periods increased, the fresh weight also increased rapidly. After six weeks of cultivation, shoots were emerged with 10.4 ea per plantlet, resulting in developing a complete plant. As a result, the bioreactor culture system for mass propagation of strawberry is required to continuously supply the air by 0.2 vvm speed and cultivate at least for six weeks.
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