Comparative Genetic Characterisitics of Korean Ginseng using DNA Markers
2013
Shin, M.R., RDA, Eumseon, Republic of Korea | Jo, I.H., RDA, Eumseon, Republic of Korea | Chung, J.W., RDA, Suwon, Repubic of Korea | Kim, Y.C, RDA, Eumseon, Republic of Ko | Lee, S.H., RDA, Eumseon, Republic of Korea | Kim, J.W, RDA, Eumseon, Republic of Korea | Hyun, D.W., RDA, Eumseon, Republic of Korea | Kim, D.H., RDA, Eumseon, Republic of Korea | Kim, K.G., RDA, Eumseon, Republic of Korea | Moon, J.Y, MIFAFF, Seoul, Republic of Korea | Noh, B.S., Seoul Womens University, Seoul, Republic of Korea | Kang, S.T., Dankuk University, Cheonan, Republic of Korea | Lee, D.J., Dankuk University, Cheonan, Republic of Korea | Bang, k.H., RDA, Eumseon, Republic of Korea
The development of random amplified polymorphic DNA(RAPD) and Expressed sequence tag-derived simple sequence repeats(EST-SSRs) provided a useful tool for investigating Korean ginseng genetic diversity. In this study, 18 polymorphic markers( 7 RAPD and 11 EST-SSR) selected to assess the genetic diversity in 31 ginseng accessions(11 Korean ginseng cultivars and 20 breeding lines.) In RAPD analysis, a total of 53 unique polymorphic bands were obtained from ginseng accessions and number of amplicons ranged from 4 to 11 with a mean of 7.5 bands. Pair-wise genetic similarity coefficient(Nei) among all pairs of ginseng accessions varied from 4 to 11 with a mean of 7.5 bands.Pair-wise genetic similarit confficient(Nei) among all pairs of ginseng accessiions varied from 0.01 to 0.32 , with a mean of 0.11 . On the basis of the resulting data, the 31 ginseng accessions were grouped into six clusters. As a result of EST-SSR analysis, 11 EST-SSR markers detected polymorphisms among the 31 ginseng accessions and revealed 49 alleles with a mean of 4.45 alleles per primer. The polymorphism information content(PIC) value ranged from 0.06 to 0.31, with an average of 0.198. The 31 ginseng accessions were classified into five groups by cluster analysis based on Nei is genetic distances. Consequently, the results of ginseng-specific RAPD and EST-SSR markers may prove useful for the evaluation of genetic diversity and discrimination of Korean ginseng cultivars and breeding lines.
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