The Use of the Pathogen-specific Bacteriophage BCP8-2 to Develop a Rice Straw-derived Bacillus cereus-free Starter Culture
2014
Bandara, N., Chonbuk National University, Jeonju, Republic of Korea | Chung, S.J., Ewha Womans University, Seoul, Republic of Korea | Jeong, D.Y., Sunchang Research Center for Fermentation Microbes, Sunchang, Republic of Korea | Kim, K.P., Chonbuk National University, Jeonju, Republic of Korea
The purpose of this study was to develop a rice straw-derived Bacillus cereus (B. cereus)-free starter culture for traditional soybean fermented products using a B. cereus-specific bacteriophage, BCP8-2. To determine the optimal medium that supports the growth of rice straw-derived microorganisms and BCP8-2 activity, 5 different culture media were tested. The 5% ground bean (GB) medium was selected for further study. No B. cereus was detected in the BCP8-2-treated rice straw in GB medium, whereas B. cereus at a level of 107 CFU/mL was recovered in the no-phage control. The total bacterial count reached approximately 109 CFU/mL regardless of phage addition. When the 16S rRNA sequence-based microbial community was monitored using denaturing gradient gel electrophoresis (DGGE) and pyrosequencing, a similar microbial community was observed in the phage-treated and control samples. In conclusion, we demonstrate that phage can be used to prepare a rice straw-derived B. cereus-free starter culture with minimal effect on natural microflora.
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