3-Hyroxyflavone in Maturation Medium Supports In Vitro Development of Fertilized Bovine Follicular Oocytes
2014
Kim, S.W., Seoul National University School of Dentistry, Seoul, Republic of Korea | Park, J.I., Konkuk University, Seoul, Republic of Korea | Jung, Y.G., ET Biotech Ltd, Jangsu, Republic of Korea | Roh, S.H., Seoul National University School of Dentistry, Seoul, Republic of Korea
Antioxidants, as reactive oxygen species scavengers, are one of the beneficial additives in serum-free defined culture medium. In this study, three separate experiments were performed to determine the effects of 3-hyroxyflavone added to the culture medium on the developmental competence of follicular bovine oocytes during in vitro maturation (IVM) and/or in vitro culture (IVC). The rate of blastocyst developed from oocytes cultured in IVM medium with 3-hyroxyflavone was significantly higher than that from control oocytes (39.0% vs. 26.3%, p0.001), respectively. However, oocytes cultured in the medium with addition of 3-hyroxyflavone only at IVC period did not show significance in the blastocyst development when compared with control. When 3-hyroxyflavone was added to both IVM and IVC media, the rate of blastocyst formation was even significantly lower (21.1%) than control (26.5%; p0.05). The present findings suggested that antioxidative activity of 3-hydroxyflavone added to only IVM medium beneficially affected the developmental competence of follicular bovine
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