Comparison of different solvents for isolation of phenolic compounds from horseradish (Armoracia rusticana L.) leaves
2013
Tomsone, L., Latvia Univ. of Agriculture, Jelgava (Latvia) | Kruma, Z., Latvia Univ. of Agriculture, Jelgava (Latvia)
Horseradish (Armoracia rusticana L.) is a perennial herb belonging to the Brassicaceae family and contains biologically active substances. The aim of the current research was to determine the most suitable extraction method and solvent for obtaining horseradish leaf extracts with high antiradical activity. For experiments fresh leaves of horseradish were extracted with seven different solvents: n-hexane, diethyl ether, 2-propanol, acetone, ethanol (95%), ethanolwater/acetic acid (80/20/1 v/v/v) and ethanol / water (80/20 by volume) using two extraction methods (conventional and Soxhlet). For all extracts, total phenolic, flavonoid content and DPPH˙, ABTS radical scavenging activity, and reducing power were determined using a spectrophotometric method. As the best solvent can be used ethanol. Total phenolic content and total flavonoid content was higher in Soxhlet extracts. Comparing to other plants, the proportion of flavonoids in the amount of total phenolics is average, and it increases by increasing the polarity of used solvent. It can be concluded that by using Soxhlet extraction method it is possible to obtain extracts that are effective antioxidants. A very strong and a strong correlation has been identified between levels of phenolic compounds and antioxidant capacities of the extracts.
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