In vitro callus and cell cultures of Eurycoma longifolia Jack as affected by types of explants
2000
Chan, L.K. | Luthfi, A.M. Siregar | Chris Teo, K.H.
Eurycoma longifolia Jack callus could be induced from its petiole, leaf and stem tissues when cultured in Murashige and Skoog (MS) medium containing different concentrations of naphthaleneacetic acid (NAA)(0-10 mg/l) and benzyladenine (BA)(0-10 mg/l). Highest callus production was obtained from petiole explant when cultured in MS medium + 2 mg/l NAA + 2 mg/l BA. Optimum growth of callus could be induced from leaf explants when cultured in MS medium + 10 mg/l NAA while stem tissues could produced the most callus in MS medium + 10 mg/l NAA + 2 mg/l BA. Calluses that were induced from petiole and stem tissues were yellowish green and compact in nature while the leaf explants produced loose and friable pale yellow callus. The production of callus became constant or reduced after the fifth or sixth subcultures. Cell cultures were established from the loose and friable callus induced from the leaf tissues. The cell suspension cultures were maintained in MS medium + 0.5 mg/l NAA
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