Gene expression profiling of Clarias gariepinus fed with omega -3 enriched pellet and non-omega-3 enriched pellet
2009
Shahreza Md. Sheriff | Fara Nor Asmira Jaslan
A study was done to determine the gene expression profiling inClarias gariepinus fed with omega-3 enriched (Diet 03) and non omega-3 enriched (Diet N03) pellet. A total of 5 samples of Asian catfish, Clarias gariepinus fed with diet 03 and diet N03_were collected. The results of the experiment showed that there wasa difference in the gene expression profiling between C. gariepinus fed with diet 03 and diet N03_when amplified using Arbitrary Primer ACP2, ACP4, ACP5, ACP8,ACP7, ACP11, ACP17 and ACP20. Range of DNA fragments obtained from the experiment were within 100 bp to 1300 bp. Overall observation found that several genes have been down-regulated in C. gariepinus fed with diet 03 as it contained lessDNA fragments compared to C. gariepinus fed with diet N03. It was also found that Primer ACP 20 produced thicker DNA fragment in Clarias gariepinus fed with diet 03 compared to Clarias gariepinus fed with diet N03 at 500 bp suggesting that the gene was highly expressed in Clarias gariepinus fed with diet 03. The study showed that a number of genes were differentially regulated by different type of nutrient. It can be stated that the gene was highly expressed in Clarias gariepinus fed with diet 03. Thestudy has shown that the supplement of omega-3 in fish feed can alter the expressed genes in fish.
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