Molecular cloning and expression levels of the monoterpene synthase gene (ZMM1) in Cassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.)
2014
Bua-In, Saowaluck (Kasetsart University, Bangkok (Thailand). Faculty of Agriculture, Department of Horticulture) | Paisooksantivatana, Yingyong (Kasetsart University, Bangkok (Thailand). Faculty of Agriculture, Department of Horticulture) | Weimer, Bart C. (University of California, Davis (USA). School of Veterinary Medicine) | Chowpongpang, Srimek (Kasetsart University, Bangkok (Thailand). Faculty of Agriculture, Department of Plant Pathology)
Cassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.) is a native Thai herb with a high content and large variety of terpenoids in its essential oil. Improving the essential oil content and quality of cassumunar ginger is dif- ficult for a breeder due to its clonally propagated nature. In this research, we describe the isolation and expression level of the monoterpene synthase gene that controls the key step of essential oil synthesis in this plant and evaluate the mechanical wounding that may influence the transcription level of the monoterpene synthase gene. To isolate the gene, the selected clones from DNA derived from young leaves were sequenced and analyzed and the monoterpene synthase gene from cas- sumunar ginger (ZMM1) was identified. The ZMM1 CDS containing 1 773 bp (KF500399) is predicted to encode a protein of 590 amino acids. The deduced amino acid sequence is 40-74% identical with known sequences of other angiosperm monoterpene synthases belonging to the isoprenoid biosynthesis C1 superfamily. A transcript of ZMM1 was detected al- most exclusively in the leaves and was related to leaf wounding. The results of this research offer insight into the control of monoterpene synthesis in this plant. This finding can be applied to breeding programs or crop management of cassumunar ginger for better yield and quality of essential oil.
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