Crude Oil-polluted Soil Induces Ultrastructural and Enzyme Activity Changes in the Shoot of Lentil
2014
Minai-Tehrani Dariush | Mohammadi Mina Kolahdouz
Plants are always under threat due to various biotic and abiotic stresses. They respond to such threats by an efficient antioxidative enzyme system such as catalase. The presence of petroleum in soil is a stressful environmental factor for plants. In this study, cellular and biochemical changes were investigated in the shoot of lentil grown in the crude oil-polluted soil. Transmission electron microscope micrographs showed various injuries in the level of subcellular content including nucleus, chloroplast and mitochondria. In the treated samples, catalase activity and its kinetics factors (Vmax and Km) were different from the control. In the treated, Km of the enzyme was lower than that of the control. Temperature and pH profiles of the enzyme were different in the control and treated samples. Catalase purification in both treated and control samples showed that two catalase isoforms were expressed in the treated sample in comparison to the control, in which only one type of catalase was detected.
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