Evaluation of the Effect of Oxidative Stress on Roots of Red Beet (Beta vulgaris L.)
2014
Kolesnikova E.V. | Ozolina N.V. | Nurminsky V.N. | Nesterkina I.S. | Sitneva L.A. | Lapteva T.I.
In the present investigation it was studied the effect of oxidative stress on root of red beet. The degree of stress exposure was assessed at various levels: in tissues of roots (conductometric method), on isolated vacuoles (time-lapse video recording), and transport activity of the vacuolar membrane enzyme, namely V-H+-ATPase (fluorescent probe method). The obtained results allow to conclude the negative effects of oxidative stress on different levels of plant organization. There were several effects: a significant increase of outflow of electrolytes from the tissues of roots, a great decrease of half-life time of isolated vacuoles, and a 2 times reduction of transport activity of tonoplast H+-ATPase. Thus, the methods used in this study can be applied to assess the intensity of the impact of oxidative stress on plants.
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