Cumulus cell expansion, its role in oocyte biology and perspectives of measurement: A review
2014
Nevoral, J., Czech University of Life Sciences, Prague (Czech Republic). Dept. of Veterinary Sciences | Orsak, M., Czech University of Life Sciences, Prague (Czech Republic). Dept. of Chemistry | Klein, P., Charles University in Prague, Pilsen (Czech Republic). Biomedical Centre | Petr, J., Institute of Animal Science, Prague-Uhrineves (Czech Republic) | Dvorakova, M., Czech University of Life Sciences, Prague (Czech Republic). Dept. of Veterinary Sciences | Weingartova, I., Czech University of Life Sciences, Prague (Czech Republic). Dept. of Veterinary Sciences | Vyskocilova, A., Czech University of Life Sciences, Prague (Czech Republic). Dept. of Veterinary Sciences | Zamostna, K., Czech University of Life Sciences, Prague (Czech Republic). Dept. of Veterinary Sciences | Krejcova, T., Czech University of Life Sciences, Prague (Czech Republic). Dept. of Veterinary Sciences | Jilek, F., Czech University of Life Sciences, Prague (Czech Republic). Dept. of Veterinary Sciences
Cumulus expansion of the cumulus-oocyte complex is necessary for meiotic maturation and acquiring developmental competence. Cumulus expansion is based on extracellular matrix synthesis by cumulus cells. Hyaluronic acid is the most abundant component of this extracellular matrix. Cumulus expansion takes place during meiotic oocyte maturation under in vivo and in vitro conditions. Quantification and measurement of cumulus expansion intensity is one possible method of determining oocyte quality and optimizing conditions for in vitro cultivation. Currently, subjective methods of expanded area and more exact cumulus expansion measurement by hyaluronic acid assessment are available. Among the methods of hyaluronic acid measurement is the use of radioactively labelled synthesis precursors. Alternatively, immunological and analytical methods, including enzyme-linked immunosorbent assay (ELISA), spectrophotometry, and high-performance liquid chromatography (HPLC) in UV light, could be utilized. The high sensitivity of these methods could provide a precise analysis of cumulus expansion without the use of radioisotopes. Therefore, the aim of this review is to summarize and compare available approaches of cumulus expansion measurement, respecting special biological features of expanded cumuli, and to suggest possible solutions for exact cumulus expansion analysis.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Library of Antonin Svehla