Relationship between hypo-osmotic swelling response of spermatozoa with post-thaw motility in crossbred bulls
2011
Chaudhari, C.F. | Prajapati, K.R. | Patel, D.M. | Patel, V.M. | Patel, T.J. | Chaudhari, A.S. | Sipai, R.S.
The relationship between hypo-osmotic swelling test with post-thaw motility in crossbred bulls was studied. The samples with 50% (110) and 60% (43) PTM were examined for HOST. Sperms with various types of curling patterns were considered as reacted and sperms with no swelling/curling were considered as non-reacted. With higher motility the HOS reacted sperms were increased in count. Positive correlation between the % of post thaw motility of spermatozoa and the spermatozoa that reacted to the hypoosmotic swelling test was observed. In addition to post-thaw motility, hypo-osmotic swelling test can be used as a regular mbase test to judge the best quality cryo-preserved semen for artificial insemination programme. Further studies are required to find out the relationship of these two tests with the field fertility.
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