Evaluation of culture, biochemical and physicochemical factors in used serums in preparation of media for Theileria annulata culture and evaluation of grown cells in bovine theileriosis vaccine.
2010
Bozorgi, Soghra | Habibi, Gollam Reza | Esmaeil nia, Kasra | Eshaghi, Ali | Hashemllo, Mosa | Negarin m ozafariyan , Zahra | Morovati, Hassan
Bovine Theileriosis Vaccine products from growth of cells infected monocytes -macrophages Theileria annulata schizonts which to grow these cells in cell culture is used inactived bovine serum as the important element . so study of factors associated with serum its growth rate and viability of cells grown in serum can be used to obtain useful information about the vaccine may eventually standardize it. In study of the factors measured included protein, sodium, potassium, glucose, cholesterol, triglycerides, and hemoglobin levels in serum samples from 12 batch significant difference wasn t seen . Also Pyrogenic test results show that all samples were free of endotoxin. Investigation performed at the Virology and Microbiology, viruses BVD, BLV and the IBR as well as Mycoplasma wasn t isolated from any of the samples. In the final passage of the 36th batch of vaccine produced in 18 monthsall cases except one passage, the cells grown in medium containing serum were in the best conditions for growth and for harvest the vaccine were in the most desirable conditions. Due to the use of fetal bovine serum is expensive bovine serum used to produce the vaccine has suitable elements . used to produce the vaccine.
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