Evaluation of polymorphism in S1 gene of avian infectious bronchitis virus in domestic isolates and vaccine using nucleotide sequencing.
2009
Hashemzadeh, Masoud | Godarzi, Hossein | Bozorgmehri, Mohammad Hassani | Masoudi, Shahiene | Karim, Vahid | Rajab, Abolfazle | Nazeme Shirazi, Mohammad Hossien | Momayez, Reza | Hassan zade, Reza | Farshiyan, Masume
Infectious bronchitis is an acute, highly contagious, viral disease of poultry with worldwide distribution, and is continuously evolving through point mutation and recombination of their genome; subsequently the emergence of IBV variants complicates disease control. The objective of this study was to investigate genetic characterization of new IBV variants isolated from commercial chicken flocks in Iran collected between 2009 and 2010. The partial S1 gene of the spike protein, covering a hypervariable and constant regions, was amplified and sequenced using conventional RT-PCR. Phylogenetic analysis revealed four viruses designated as Razi-HKM891, Razi-HKM892, Razi-HKM893 and Razi-HKM894. Deduced amino acid sequence comparison with other IBV genotypes, published in the GenBank database, indicated that the isolates Razi-HKM891 and Razi-HKM894 were placed into the pathogenic 4/91. However, the isolates Razi-HKM892 and Razi-HKM893 were different with previously described isolates in Iran. The Razi-HKM893 is closely related to recently published isolates from Occupied Palestine (IS/720/99, IS/885) and Iraq (Sul/01/ 09), 96.5 % and 97.7 % amino acid sequence identities respectively. These isolates, included in the nephropathogenic IBV strains, belong to a new genotype in Middle East and likely indigenous to Iran. Key words: Infectious bronchitis (IB); Phylogenetic analysis; Amino acid sequence; Variants.
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