An efficient transformation method for a potato (Solanum tuberosum L. var. Atlantic)
2015
Han, E.H., Dept. of Agronomy and Medicinal Plant Resources, College of Life Science, Gyeongnam National University of Science and Technology, Jinju, Republic of Korea | Goo, Y.M., Sancheong Oriental Medicinal Herb Institute, Sancheong, Republic of Korea | Lee, M.K., Dept. of Agronomy and Medicinal Plant Resources, College of Life Science, Gyeongnam National University of Science and Technology, Jinju, Republic of Korea | Lee, S.W., Dept. of Agronomy and Medicinal Plant Resources, College of Life Science, Gyeongnam National University of Science and Technology, Jinju, Republic of Korea
We found that a long period of in vitro culture is a critical factor on the low transformation rate for a specific potato genotype, Solanum tuberosum L. var. Atlantic when phosphinothricin (PPT) was added to select putative transformants in a solid media. The fresh explants of the newly produced plants from a micro - tuber was able to increase the transformation rate significantly while the old explants prepared from a plant maintained for longer than 6 months in vitro by sub-culturing every 3 ~ 4 weeks resulted in a very low transformation frequency. However, Jowon cultivar was not so much influenced by the period of in vitro culture with high transformation rate (higher than 10.0 %). Further research need to be explored for the reason why a particular potato genotype, Atlantic is more vulnerable than the Jowon cultivar during the regeneration stage resulting in the low transformation frequency.
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