Rapid comparison of metabolic equivalence of standard medicinal parts from medicinal plants and their in vitro-generated adventitious roots using FT-IR spectroscopy
2015
Ahn, M.S., Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB), Daejeon, Republic of Korea | Min, S.R., Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB), Daejeon, Republic of Korea | Jie, E.Y., Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB), Daejeon, Republic of Korea | So, E.J., Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB), Daejeon, Republic of Korea | Choi, S.Y., Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB), Daejeon, Republic of Korea | Moon, B.C., K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea | Kang, Y.M., K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea | Park, S.Y., Department of Horticultural Science, College of Agriculture, Life and Environment Science, Chungbuk University, Cheongju, Republic of Korea | Kim, S.W., Microbial Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea
To determine whether metabolite fingerprinting for whole cell extracts based on Fourier transform infrared (FT-IR) spectroscopy can be used to discriminate and compare metabolic equivalence, standard medicinal parts from four medicinal plants (Cynanchum wilfordii Hemsley, Atractylodes japonica Koidz, Polygonum multiflorum Thunberg and Astragalus membranaceus Bunge) and their in vitro-produced adventitious roots were analyzed by FT-IR spectroscopy. The principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) from the FT-IR spectral data showed that the whole metabolic pattern from Cynanchum wilfordii was highly similar to Astragalus membranaceus. However, Atractylodes japonica and Polygonum multiflorum showed significantly different metabolic patterns. Furthermore, adventitious roots from Cynanchum wilfordii and Astragalus membranaceus also showed similar metabolic patterns compared to their standard medicinal parts. These results clearly show that mass proliferation of adventitious roots may be applied to aquire novel supply of standard medicinal parts from medicinal plants. However, the whole metabolic pattern from adventitious roots of Atractylodes japonica and Polygonum multiflorum were not similar to their standard medicinal parts. Furthermore, FT-IR spectroscopy combined with multivariate analyses established in this study may be applied as an alternative tool to discriminate the whole metabolic equivalence from several standard medicinal parts. Thus, we suggest that these metabolic discrimination systems may be applied for metabolic standardization of herbal medicinal resources.
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