Antioxidant Activity of the Halophyte Ligustrum japonicum
2015
Baek, S.O., Korea Maritime and Ocean University, Busan, Republic of Korea | Kim, H., Korea Maritime and Ocean University, Busan, Republic of Korea | Jeong, H., Korea Maritime and Ocean University, Busan, Republic of Korea | Ju, E., Korea Maritime and Ocean University, Busan, Republic of Korea | Kong, C.S., Silla University, Busan, Republic of Korea | Seo, Y., Korea Maritime and Ocean University, Busan, Republic of Korea
Dried samples of Ligustrum japonicum were extracted twice: with methylene chloride and with methanol (MeOH), respectively. The combined crude extracts were successively fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions by liquid-liquid partition. Antioxidant activities of crude extract and its solvent fractions were evaluated by measuring authentic ONOO-, and ONOO- generated from 3-morpholinsydnonimine (SIN-1) as well as degree of occurrence of intracellular ROS in HT 1080 cells, and genomic DNA oxidation. The 85% aq.MeOH and n-BuOH fractions exhibited the good antioxidant activity. Further purification of the 85% aq.MeOH fracition led to the isolation of Oleanolic acid (1), Maslinic acid (2), and Ursolic acid (3). All compounds showed the significant antioxidant effects in all assay systems.
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