Method Development and Analysis of Carotenoid Compositions in Various Tomatoes
2015
Kim, H.K., Chungnam National University, Daejeon, Republic of Korea | Chun, J.H., Chungnam National University, Daejeon, Republic of Korea | Kim, S.J., Chungnam National University, Daejeon, Republic of Korea
BACKGROUND: Purpose of this research is HPLC analysis method development of lycopene in tomato. And then, three components of carotenoid in four kinds of tomatoes (general tomato, cherry tomato, red and orange date tomato) were compared with each other. METHODS AND RESULTS: Lycopene in tomato was extracted with hexane likes other carotenoid components using 500 mg of dried powder sample. HPLC analysis conditions were column temperature (40 Celsius), detection wavelength (454 nm), flow rate (1.0 mL/min) and injection volume (20.0 microliter). Lycopene was analyzed by the gradient elution (60 - 100%) of the mobile phase solvents A[water: methanol=25: 75 (v/v)] and B[ethyl acetate]. CONCLUSION: Three components of carotenoids (lutein, beta-carotene, lycopene) were observed in tomatoes. The total carotenoid contents was the highest in red date tomato (662.0 mg/kg dry wt.) and the lowest in orange date tomato (111.3 mg/kg dry wt.). Lycopene contents in tomatoes was the highest percentage (93%) among all the carotenoids.
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