Synthesis of Hapten for Indirect Competitive Immunoassay for Measuring 3,5,6-trichloro-2-pyridinol
2015
Kim, A., National Institute of Agricultural Sciences, Wanju, Republic of Korea | Kim, J.Y., BIONAST Co., Ltd., Seongnam, Republic of Korea | Jeong, S.H., Hoseo University, Asan, Republic of Korea | Cho, M.H., Seoul National University, Seoul, Republic of Korea | Park, K.H., Seoul National University, Seoul, Republic of Korea | Cho, N., National Institute of Agricultural Sciences, Wanju, Republic of Korea | Paik, K., National Institute of Agricultural Sciences, Wanju, Republic of Korea
BACKGROUND: In this study, we have attempted to identify a urinary biomarker to assess chlorpyrifos exposure in farmers. The major metabolite and the excretion pathway of chlorpyrifos is 3,5,6-trichloro-2- pyridinol (TCP) in urine. Herein, we describe an adequate synthetic method for TCP hapten for measuring urinary TCP of farmers. METHODS AND RESULTS: First, TCP was prepared by spacer attachment through hydrolysis of thiophosphate ester from chlorpyrifos. After reaction with benzyl bromide, the TCP was transformed into 2,3,5-trichloro-6- benzyloxypyridine. Next, the chlorine in the 2nd position of the pyridyl ring was substituted into 3-mercaptopropanoic acid spacer arm. Finally, the phenyl group attached to the 6th position in pyridyl ring was removed for producing the targeted product, 3-(3,5-Dichloro-6-hydroxy-2-pyridyl) thiopropanoic acid. CONCLUSION: Henceforth, this TCP hapten would be used in developing immunoassay studies for the detection and quantitation of urinary TCP of farmers.
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