Mass-production of Four Specific Proteins Originated from Deformed Wing Virus, Honeybee-viral Pathogen
2015
Lee, J.S., Kyonggi University, Suwon, Republic of Korea | Luong, G.T.H., Kyonggi University, Suwon, Republic of Korea | Yoon, B.S., Kyonggi University, Suwon, Republic of Korea
Deformed Wing Virus (DWV) is one of viral pathogens in honeybee and caused a wing deformity and a premature death of honeybee. In this study, capsid protein VP1, capsid protein VP1-VP3 complex, RNA dependent RNA Polymerase (RdRP) and C3G peptidase (C3G) originated from DWV were successfully over-expressed using E. coli expression system, respectively. Each recombinant proteins using pET32a(+) or pMAL-C2 system was purified using affinity-column chromatography under optimum expression conditions, Four specific proteins in this study would be use as antigens to generate specific antibodies against DWV. Especially, recombinant RdRP and recombinant C3G might be useful materials to studies on enzymatic analysis and inhibitory assay of DWV.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Korea Agricultural Science Digital Library