Genetic improvement of upland rice through marker assisted selection (MAS) for tolerance to phosphorus deficiency
2015
Lapitan, V.C. | Mercado, M.J.T.
Phosphorus (P) deficiency is a major abiotic stress that limits plant growth and yield in upland rice production. The development of phosphorus uptake (Pup1) rice cultivars may be an attractive and cost effective approach to increasing rice yields where P deficiency is the major constraint. Pup1 is a major quantitative trait locus (QTL) located on rice chromosome 12 which confers tolerance to P deficiency in soil. The study aimed to develop elite breeding lines for upland condition with yield higher than the current level and tolerant to P-deficient soils. A parental survey of 93 rice germplasm using Pup1 markers such as K20-2, K29-1, K29-3, K46-1, was conducted to identify potential parents and to plan possible combinations. NSIC Rc240, NSIC Rc238, NSIC Rc222 and PSB Rc82 were identified to be the recipient parents while Vandana was selected as the donor parent. Utilizing the selected parents, four cross combinations were generated. Foreground selection using Pup1 marker (K29-1) on the 21 day-old F1 seedlings produced from the four crosses revealed that Pup1 gene was present. The F1 materials carrying Pup1 gene were utilized accordingly in the generation of different elite lines such as near isogenie lines (NILs), recombinant inbred lines (RILs) and doubled haploid lines (DHLs). For the development of such lines, marker assisted selection was being employed using Pup1 markers for foreground selection and closely linked SSR markers for background selection. To date, these breeding lines were on the advanced stage and are planted in the field for performance evaluation.
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