Screening commercial wheat (Triticum aestivum L.) varieties for agrobacterium mediated transformation ability
2016
Abid, N. (Forman Christian Coll., Lahore (Pakistan). Dept. of Biological Sciences) | Maqbool, A. (Forman Christian Coll., Lahore (Pakistan). Dept. of Biological Sciences) | Malik, K.A. (Forman Christian Coll., Lahore (Pakistan). Dept. of Biological Sciences)
Wheat is staple food crop of many countries including Pakistan. It has a large number of cultivars and genotypes. All genotypes have different tissue culture response that includes callus induction, regeneration and transformation efficiency. For transgenic plant production it is crucial to know tissue culture efficiency of a selected variety. Therefore, in the present study mature embryos of thirteen elite wheat (Triticum aestivum L.) varieties were evaluated for tissue culture response and their amenability to transformation. Each variety responded differently for callogenesis, transient GUS (beta-glucuronidase) expression and regeneration. The results for callus induction and transient GUS expression ranged from 30-100% and 13-100%, respectively whereas regeneration response was quite different in tested varieties that ranged from 0-44%. Good quality callus was observed in all varieties except Dhurabi-11, Lasani-08, Millat & Pak-81. Maximum transient GUS expression (100%) was found in Faisalabad-2008. Highest regeneration (44%) was noticed in Pak-81. Results indicated that three varieties VIII-83, Faisalabad- 2008 and Aas-11 are suitable for transformation in comparison to others.
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