Effect of culture media and growth regulators on in vitro propagation of Chrysanthemum indicum L.
2016
Zafarullah, A. (Lahore Coll. for Women Univ., Lahore (Pakistan). Dept. of Biotechnology) | Ilyas, S. (Lahore Coll. for Women Univ., Lahore (Pakistan). Dept. of Biotechnology) | Naz, S. (Lahore Coll. for Women Univ., Lahore (Pakistan). Dept. of Biotechnology) | Aslam, F. (Lahore Coll. for Women Univ., Lahore (Pakistan). Dept. of Biotechnology) | Manzoor, F. (Lahore Coll. for Women Univ., Lahore (Pakistan). Dept. of Zoology)
A rapid shoot multiplication of Chrysanthemum (Chrysanthemum indicum L.) has been optimized in the present study. Apical meristems were used as explants and inoculated on MS media supplemented with various concentrations of growth regulators. The effects of different phytohormones (BAP, NAA, IAA and IBA) were tested for their effect on enhancement of shoot multiplication. MS medium fortified with BAP 1.0 + IAA 0.1 mg/l had a promotory effect on the initiation and multiplication of micro shoots. The optimum shoot formation response was 82% with 5.20 average number of shoots per explants and acquired maximum length of 4.9 cm. Rooting study revealed that MS medium supplemented with IBA 0.1 mg/l were proved to be superior with maximum (85%) rooting, 2.7 roots per explants and 2.5 cm root length. Then plantlets were shifted in Green house for acclimatization.
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