Salt Tolerance Evaluation in Cotton (Gossypium hirsutum L.) Using RAPD Marker
2015
Saleh Basel
The aim of this study was to evaluate four upland cotton (Gossypium hirsutum L.) [Niab78 (N78), Deir-Ezzor22 (DE22), Deltapine50 (DP50) and Aleppo118 (A118)] varieties towards salt stress (0 and 200 mM NaCl) for 7 weeks based on RAPD marker. Our data showed that the highest total polymorphic bands identified by the 26 RAPD tested primers were 150 bands generated by N78 variety, while the lowest ones were recorded for DP50 (29 bands). Otherwise, unique (negative and positive) markers characterized the two tolerant varieties (N78 and DE22) were 22 and 29 markers, respectively. Our data indicated that the highest polymorphism level was detected in N78 variety (68.5%) followed by DE22 (60.9%), whereas, the lowest one was recorded for DP50 (21.3%). Our data obtained herein indicates that RAPD marker provided molecular markers for salinity tolerance screening in early stage in genetic improvement programs.
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