Kinetic studies of goat milk lactoperoxidase
2015
Kakhanouskaya,E.Yu. | Semak,I.V.
English. Lactoperoxidase (LPO) is an oxidoreductase secreted into milk, and playing an important role in protecting the lactating mammary gland and the intestinal tract of the newborn infants against pathogenic microorganisms. A large number of different peroxidases have been purified and characterized, but kinetic parameters of goat lactoperoxidase are still uncertain. The aim of the study presented in the article was to determine the kinetic parameters of goat milk LPO. Km values at optimum pH were 0.084mM, 2.1mM, 0.75mM for SCN-, Br- and I-, respectively Kkat were 12.5 c-1 and 0.72 c-1 for SCN- and Br-, respectively. Kkat/Km were 1.5x10*5 M-1c-1 and 3.8x10*2 M-1c-1 for SCN and Br-, respectively. LPO can be used as a biopreservative agent in food, feed specialties, cosmetic and related products.
Show more [+] Less [-]Russian. Установлены зависимости начальных скоростей окисления субстрата от концентрации лакто-пероксидазы (ЛПО) молока козы и от рН, определены оптимум рН для окисления естественных субстратов ЛПО (SCN- Br-, I-), а также кинетические характеристики данного фермента. Полученные данные кинетических параметров ЛПО молока козы можно использовать в технологических процессах для контроля качества с целью предотвращения деструкции ферментов и ингиби-рования их активности при производстве продуктов питания и косметических препаратов.
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