Reversed-phase high-performance liquid chromatography analysis of β-lactoglobulin and α-lactalbumin in different types of milk | Dažādu piena paraugu analīze apgrieztās fāzes augstas efektivitātes šķidruma hromatogrāfijas apstākļos
2016
Rotkaja, O., University of Latvia, Riga (Latvia) | Golusko, J., Institute of Chromatography, Riga (Latvia) | Mekss, P., University of Latvia, Riga (Latvia)
English. The aim of this study was to detect major whey proteins α-lactalbumin and β-lactoglobulin by reversed-phase high-performance liquid chromatography (RP-HPLC). RPHPLC methods were developed using the column YMC Pack-C4, which enabled the separation of whey proteins within 30 min. Mobile phase was acetonitrile/water/0.1% trifluoroacetic acid (TFA) with gradient elution, flow rate was 1.0 mL·minE−1, and the detection wavelength was 210 nm. Column temperature was 40°C and injection volume was 20 μL. Milk samples contained α-lactalbumin and total β-lactoglobulin: No. 1 - 1500 mg·LE−1 and 3600 mg·LE−1; No. 2 - no α-lactalbumin and 450 mg·LE−1 total β-lactoglobulin; No. 3 - 800 mg·LE−1 and 94 mg·LE−1, respectively.
Show more [+] Less [-]Latvian. β - laktoglobulīnu var izmantot kā indikatoru, lai noskaidrotu kāds pasterizācijas veids ir izmantots pienam. Šī darba ietvaros, izmantojot α - laktalbumīnu, β - laktoglobulīnu A un B standartvielu maisījumu, tika atrasti hromatogrāfiskie apstākļi olbaltumvielu atdalīšanai. Tika izvēlēta kolonna C4, ar kuru tika veikta sākotnējo hromatogrāfisko apstākļu optimizācija, lai iegūtu pilnīgu olbaltumvielu atdalīšanu. Tika izstrādāta metode uz C4 kolonnas par kustīgo fāzi lietojot acetonitrila/ūdens /0,1 % TFA maisījumu, analīzes laiks ir 30 min.
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