Development of live vaccines from Yersinia ruckeri by aroA and aroC gene mutations
2013
Altinok, Ilhan (Karadeniz Technical University, Trabzon (Turkey). Faculty of Marine Sciences) | Kahraman, Umit (Karadeniz Technical University, Trabzon (Turkey). Faculty of Marine Sciences) | Capkin, Erol (Karadeniz Technical University, Trabzon (Turkey). Faculty of Marine Sciences) | Boran, Halis (Karadeniz Technical University, Trabzon (Turkey). Faculty of Marine Sciences) | Ozturk, Rafet (Karadeniz Technical University, Trabzon (Turkey). Faculty of Marine Sciences)
Yersiniosis caused by Yersinia ruckeri is the main causes of high mortalities and severe economic losses in freshwater and marine aquaculture. To prevent this disease, a live attenuated vaccine was developed. For this purpose, Y. ruckeri aroA and aroC genes were amplified and DNA fragments mutated by overlap extension PCR was cloned into a suicide plasmid. This plasmid was transferred to Y. ruckeri for replacing wild type genes with mutated aroA and aroC genes via homologous recombination. Virulence of mutant Y. ruckeri was determined by infecting juvenile rainbow trout with mutant bacteria and calculating fish mortalities and immune responses in fish. Similarly, efficacy of mutant Y. ruckeri was determined by vaccinating fish with mutant bacteria and then challenging the same fish with wild type Y. ruckeri RB0708 6 months after vaccination. Vaccination of rainbow trout with the aroA and aroC mutant as a live vaccine conferred significant protection against the wild-type Y. ruckeri.
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