Computational studies on LiP H isolated from Ganoderma lucidum GD88
2015
Parambayil, Nayana (Cochin University of Science and Technology, Cochin (India). Department of Biotechnology, Plant Biotechnology Laboratory) | Chenthamarakshan, Aiswarya (Cochin University of Science and Technology, Cochin (India). Department of Biotechnology, Plant Biotechnology Laboratory) | Anto, Arinnia (Cochin University of Science and Technology, Cochin (India). Department of Biotechnology, Plant Biotechnology Laboratory) | Hariharan, Sudha (Cochin University of Science and Technology, Cochin (India). Department of Biotechnology, Plant Biotechnology Laboratory) | Nambisan, Padma (Cochin University of Science and Technology, Cochin (India). Department of Biotechnology, Plant Biotechnology Laboratory)
Ganoderma lucidum is a basidiomycete fungus that produces ligninase for the modification of lignin. Lignin peroxidase (LiP) is a glycoprotein that acts on the recalcitrant cell wall component lignin. In the present study, the phylo- genetic analysis of Ganoderma lucidum GD88 with the partial coding sequence (cds) of other LiP isoforms was performed using MEGA6. After determination of the open reading frame, the +3 frame nucleotide sequence was converted to protein using the EMBOSS Transseq and the secondary structure was predicted using the Chou and Fasman Secondary Structure Prediction server (CFSSP). Protein modeling was also performed by SWISS-MODEL. The obtained result shows that the lipH partial cds of Ganoderma lucidum GD88 is homologous to the lipD gene of Phanerochaete chrysosporium. The sec- ondary structure prediction result revealed that the percent content of the helix (67) is higher than the percent contents of sheet (53.4) and turns (13.6). According to the generated model, LiP H protein is a homodimer with chains A and B. The heme acts as a ligand and plays a major role in structure stabilization.
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