Biochemical characterization based on SDS-PAGE analysis and correlation among traits in opium poppy (Papaver Somniferum L.) germplasm
2015
Verma, Nidhi (National Botanical Research Institute, Lucknow (India). Genetics and Plant Breeding) | Shukla, Sudhir (National Botanical Research Institute, Lucknow (India). Genetics and Plant Breeding) | Yadav, Kusum (University of Lucknow, Lucknow (India). Department of Biochemistry) | Mishra, Brij K. (National Botanical Research Institute, Lucknow (India). Genetics and Plant Breeding) | Rastogi, Anu (National Botanical Research Institute, Lucknow (India). Genetics and Plant Breeding)
This research aimed to investigate the genetic diversity based on the pattern of seed storage protein and to establish correlation between alkaloid and seed protein content among 124 germplasm lines of opium poppy (Papaver somniferum L.). Twenty seven polypeptide bands in range of 6 to 205 kDa were recorded. Similarity index was calculated by using Jaccard’s Similarity index and cluster analysis was performed using UPGMA model. Polymorphism was observed in three variable regions i.e., high, medium and low molecular weight, among which bands of 10.4, 20, 22, 24, 30, 32, 33, 49 and 205 kDa’s were common in all the germplasms but other bands showed variation. All the 124 germplasms were broadly grouped into 13 clusters based on average linkage method. None of the trait showed significant correlation with seed protein content. The differential protein profile of the germplasms revealed wide variability and diversity among the studied germplasms which could be further used in hybridization programme to obtain maximum gain.
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