Micropropagation of oncidium sp. by plant tissue and cell culture techniques
2014
Nguyen Van Khoa;Tran Van Minh
High demand of Oncidium orchid leading to find out the efficient methods for propagation. Traditional propagation rate was low in the nature and hybrid seeds were not stable genetically. Plant cell biotechnology was the efficient ways to resolve the barrier of elite clones production. MS medium supplemented with 2.4D (1 mg/l) was favored for callus induction (76.19%). In the same MS medium supplemented with NAA (0.75 mg/l) was favored for PLB regenerated (98 PLB/callus cluster) from callus cultivation; supplemented with combination of BA (0.5 mg/l) and NAA (0.5 mg/l) was favored for PLB regenerated (28.18 PLB/shoot cluster) from shoots cultivation. The PLBs (79.21 PLB/PLB cluster) were proliferated on MS medium supplemented with combination of NAA (1 mg/l) and with BA (1 mg/l) for PLB regenerated to multiple-shoots (12.42 shoots/PLB cluster). Multiple-shoots were divided to 3-4 shoots/cluster for far micropropagation on the MS medium supplemented with combination of BA (0.25 mg/l) and NAA (0.25 mg/l) to reach 11.66 shoots/cluster. Shoots were separated to single-shoot for rooting on the MS medium supplemented with NAA (0.75 mg/l). A scheme for Oncidium micropropagation by PLB culture techniques was set up.
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