COMPARATIVE CHARACTERISTIC OF SPECTRUM BACTERIOPHAGES AGAINST BACILLUSANTHRACIS, ISOLATED FROM SAMPLES EXTERNAL ENVIRONMENT OF GEORGIA. 2-nd REPORT. B. anthracis, SPECIFIC PHASE selection in the 2010-2011
2017
Lia Gubeladze, Dali Gogiashvili, Sergo Rigvava, Merab Natidze, GiaTsertsvadze | George Kamkamidze, Marina Tediashvili
Anthrax is a potentially fatal infectious disease naturally occurring in different regions of the world. The causing agent, the spore-forming microorganism B. anthracis, can be used as a bioweapon. Multi drug resistant anthrax strains can be considered as the most significant biothreat agents. Thus, countermeasures to neutralize this threat are highly demanded. This paper describes isolation techniques and characterization of new bacteriophages active against B.anthracis. The series of experiments aiming isolation of new phage clones specific to B.anthracis have been carried out during warm season 2010-11. Five types of bacteriophages active against B.anthracis– Ba PAT, Ba ActIV, Ba ActV, Ba InsL and Ba InsZ have been isolated from environmental sources in Georgia. Initially, the phages were isolated on B. anthracis vaccine strains STI-1 and 34F2, using standard enrichment technique.TEM studies of newly isolated Ba phages have shown that the virion morphologies consistent with the Siphoviridae family of tailed bacteriophages (with isometric head and long noncontractile tail),although phages differ from each other by the size. The B.anthracis phages have been comparatively characterized based on phenotypic and genotypic properties, such asvirion and negative plague morphology The newly isolated Baphages showed also diverse serologic characteristics, with BaActIV and BaActV phages more closely related to phage Gamma. The Ba phages demonstrated broad lytic spectrum within the species, which indicates to their potential for identification and biocontrol of B.anthracis.
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